Introduction to medical laboratory technology pdf

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download Introduction to Medical Laboratory Technology - 5th Edition. Print Book & E-Book. Price includes VAT/GST. DRM-free (EPub, PDF, Mobi). × DRM-. An Introduction to Medical Laboratory Technology, Second Edition provides information pertinent to medical laboratory technology. This book discusses the. Introduction to Medical Laboratory Technology. Berhanu Seyoum. Haramaya University. In collaboration with the Ethiopia Public Health Training Initiative, The .

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Introduction To Medical Laboratory Technology Pdf

An Introduction to Medical Laboratory Technology Get a printable copy (PDF file) of the complete article (K), or click on a page image below to browse. The mission of the Medical Laboratory Technology Program is to prepare the student to . MLT INTRO TO MEDICAL LABORATORY TECHNOLOGY. BAKER AND SILVERTON'S INTRODUCTION TO MEDICAL LABORATORY TECHNOLOGY Table of Contents Preface to the Seventh Edition Preface to the Sixth.

Personal information is secured with SSL technology. Free Shipping No minimum order. Description Introduction to Medical Laboratory Technology presents the development in the medical laboratory science. It discusses the general laboratory glassware and apparatus. It addresses a more specialized procedure in mechanization, automation, and data processing. Some of the topics covered in the book are the composition of glass; cleaning of glassware; the technique of using volumetric pipettes; technique for centrifugation; the production of chemically pure water; principal foci of a converging lens; micrometry; magnification; setting up the microscope; and fluorescence microscopy. The precautions against infection are covered. The storage of chemicals and treatment of accidents are discussed. The text describes the collection and reporting of specimens.

May Learn how and when to remove this template message In most developed countries, there are two main types of lab processing the majority of medical specimens. Hospital laboratories are attached to a hospital , and perform tests on their patients. Private or community laboratories receive samples from general practitioners , insurance companies, clinical research sites and other health clinics for analysis. For extremely specialised tests, samples may go to a research laboratory. Some tests involve specimens sent between different labs for uncommon tests.

For example, in some cases it may be more cost effective if a particular laboratory specializes in a less common tests, receiving specimens and payment from other labs, while sending other specimens to other labs for those tests they do not perform. In many countries there are specialized types of Medical Laboratories according to the types of investigations carried out. Organisations that provide blood products for transfusion to hospitals, such as The Red Cross, will provide access to their reference laboratory for their customers.

An introduction to medical laboratory technology

Some laboratories specialize in Molecular diagnostic and cytogenetic testing, in order to provide information regarding diagnosis and treatment of genetic or cancer-related disorders.

Specimen processing and work flow[ edit ] In a hospital setting, sample processing will usually start with a set of samples arriving with a test request, either on a form or electronically via the laboratory information system LIS. Inpatient specimens will already be labeled with patient and testing information provided by the LIS. Entry of test requests onto the LIS system involves typing or scanning where barcodes are used in the laboratory number, and entering the patient identification, as well as any tests requested.

This allows laboratory analyzers, computers and staff to recognize what tests are pending, and also gives a location such as a hospital department, doctor or other customer for results reporting. Once the specimens are assigned a laboratory number by the LIS, a sticker is typically printed that can be placed on the tubes or specimen containers.

This label has a barcode that can be scanned by automated analyzers and test requests uploaded to the analyzer from the LIS. Specimens are prepared for analysis in various ways.

For example, chemistry samples are usually centrifuged and the serum or plasma is separated and tested. If the specimen needs to go on more than one analyzer, it can be divided into separate tubes.

Responsibility of histopath technician. Properties of fixatives, amount of fixative. Tissue Processing: Tissue processing of histological tissues for paraffin embedding. Mould - types of moulds, techniques of casting embedding media. Preparation of histopathological block. Demonstration of microtomes. Preparation of fixative. Microtome knives - General description of microtomel knives, Types of microtome knives.

Sharpening of microtome knives - 1. Honning - definition, types of hones, lubricants for hone, methods of honing 2. Stropping - definition, method of stopping. Section cutting: Process of section cutting of paraffin embedded tissue - fixing of block, trimming of tissue block, picking up sections, drying of section etc.

Types of section - serial section, step section. Trouble shooting for poor sections Discrepancies in section cutting 3. Mounting and mounting media: Introduction, Criteria of acceptable mounting media. Preparation of tissue block 0. Section cutting 0. Introduction, various terminologies.

Definition, Steps Process of decalcification, Factor affecting decalcification. Determination of end points of decalcification - various methods with procedure. Treatment of hard tissues for decalcification.

Metachromasia and metachromatic dyes. Slide preparation of histological section.

An Introduction to Medical Laboratory Technology

Mounting of slide. Harsh Mohan, Text book of pathology Suggested Reading 1.

Culling Histopathology techniques. Quantitative determination of blood sugar: Types of sample for plasma sugar estimation - Fasting blood sugar, Post pendrum and random blood sugar. Significance of fluoride as anticoagulant. Clinical significance of estimation of blood sugar. Quantitative determination of blood Lipid: Principle, procedure, calculation and clinical significance of — Triglyceride, Total cholesterol, HDL cholesterol. Blood Nitrogen: Estimation of total protein, Albumin - Principle, procedure, calculation and clinical significance.

Significance of A: G ratio. Estimation of plasma glucose fasting 0. Estimation of total protein, Albumin 0. Introduction to isoenzymes. Unit for expression of enzyme activity. Principle of assay, procedure and clinical significance of Serum glutamate oxaloacetate transaminase AST , Serum glutamate pyruvate transaminase ALT , Lactate dehydrogenase, Alkaline phosphatase and Acid phosphatase, 3. Estimation of serum SGPT estimation. Estimation of serum alkaline phosphatase.

Qualitative test of protein in urine 0. Qualitative test of ketone bodies 0. Qualitative test of bile salt 0. Qualitative test of reducing substance sugar in urine. H Talib, A hand book of medical laboratory technology. Godkar, A text book of medical laboratory technology.

Introduction to Medical Laboratory Technology

Gram staining of urine specimen. Preparation of media for urine culture. Inoculation of urine specimen on culture media. Identification of culture by colony characteristics. Microbiological examination of sputum: Microbiological examination of Pus, stool: Identification of culture by colony characteristics 5.

Biochemical identification of bacteria. Definition, general characteristics, types, hepten, carrier protein, epitopes, adjuvents. Immunoglobulin vs antibodies, structure of antibodies, paratopes, different classes and their function.

Antigen - Antibody reaction: Specificity, sensitivity and avidity. Measurement of Antigen Antibody reaction: Demonstration of qualitative agglutination reaction widal test 0.

Demonstration of blood grouping slide method 0. Demonstration of flocculation reaction RPR test. Introduction to clinical pathology and Laboratory organization and management. Composition of urine, Collection, Transport, Preservation of urine.

Physical Examination: Volume Specific gravity, Color, Turbidity, Odor etc. Chemical examination: Strip Techology. Microscopic Examination: Procedure of urine examination.

Presence of various cells, Ova and parasites, various crystals and their identification, casts-their types and identification etc.

Routine Examination of urine. Microscopic examination of urine. Qualitative test of albumin in urine 0. Test for ketone bodies 0. Seminal fluid: Introduction, Collection of semen. Physical examination: Quantity, Viscosity, Colour, Reaction and their clinical significance. Microscopic examination of semen: Sperm count, motility of spermatozoa, Morphological examination of spermatozoa, other various cells.

Introduction, Collection of specimen for stool examination. Normal appearance and composition. Amount, Form and consistency, colour, odour, reaction, mucous and their significance. Occult blood, Bilirubin, Stercobilin, urobilinogen, fat and nitrogen etc.

Concentration Techniques: Concentrated saline floatation, Formal Ether Co 5. Fuctosamin test. Microscopic examination of semen. Preparation and examination of stool. Qualitative test for occult blood in stool. F Cerebrospinal Fluid: Introduction and normal physiology of CSF. Normal values for lumbar C. F in adults: Pressure, volume, Specific gravity, various cells, Proteins and other various organic and inorganic constituents. Collection, transport and preservation of C.

Appearance and colour, Formation of clot B. Chemical Examination: Estimation of total proteins, Qualitative test for globulin, Quantitative protein estimation. Cell counts: Bacteriological examination: Physical, Chemical examination. Cytological examination - Differential cell count.

Bacteriological Examination- Gram and ZN staining on smear from the sediment, Culture on suitable media. At the completion of this year the students will be able to perform all the relevant lab tests for specific pathological tests.

Blood Banking Techniques —II: Blood 2. Blood 3 2. Typhoid, UTI 0. Preventive and Social Medicine—II: Blood group system: Rh System: Antisera used in ABO grouping procedures. Blood grouping techniques: Principal of blood grouping. Reverse blood grouping by tube method. Du test for detection of Du antigens. Blood donation: Blood Collection: Testing of Donor Blood: Screening of transfusion transmitted infection TTI.

Introduction to Medical Laboratory Technology

Bacterially contaminated blood. Storage of blood. Changes in donor blood after storage. Blood components: Collection of blood components for fractional transfusion. Cryo-preparation and its significance. Introduction 2. Compatibility Testing: Introduction, Cross matching — Types, principle, procedure and its significance. Introduction Lab.

Minor cross matching. Major cross matching. Godkar, Text book of Medical Laboratory Technology. Diabetes mellitus: Diabetes insipidus- introduction. Laboratory diagnosis of diabetes. Thyroid disorders: Thyroid finction test. Estimation of glucose in plasma 0. Determination of urinary sugar 0. Liver function test — test based on metabolic function, test based on excretory function, test based on synthetic function, enzyme estimation etc. Estimation of serum ALP 0. Estimation of SGPT.

Estimation of SGOT. Estimation of serum total bilirubin. Estimation of serum direct bilirubin. Lipid profile: Electrolytes - quantitative estimation of sodium, potassium, calcium, chloride, and their clinical significance.

Estimation of serum total cholesterol. Estimation of serum HDL cholesterol. Estimation of serum triglyceride. Estimation of serum calcium. Godkar, Text of Medical Laboratory technology. General Requirements for Collecting and Submitting Specimens: Preparation of container and swab for collection of specimens for microbial examination.

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Flowchart of diagnostic procedure in microbiology laboratory. Widal test qualitative. Montaux test 0. H7, Stool Culture - Vibrio spp. Preparation of swab 0. Culturing of blood. Culturing of pus. Culturing of sputum. Culturing of stool. Sampling Methods In Microbiology: Rapid test of VDRL. Godkar, Text book of medical laboratory technology.

C Baweja, Text book of medical laboratory technology. Reference Book 1. Water, air and noise pollution: The concept of safe and wholesome water, removal of hardness of water, methods of purification of water on small scale and large scale. Standards of Water quality. Air and noise pollution and their prevention.

Hygine and sanitation: Incineration and disinfection 3. Infection and control: Source and agent of infection in community. Epidemiology surveillance and control of infection in community infection. Methods of prevention and control- isolation of patient, quarantine and incubation period of various infectious disease.

Prophylactic Immunization: Various national immunization programs and vaccine schedule. Family welfare planning and child health care programs. Healthcare by balance diet and yoga: Normal constituents of diet, balance diet and factor responsible for etiology of various nutritional disorders. Carcinogens in food. Role of regular exercise and yoga prevention and management of various disease.

Introduction to Nursing: What is Nursing? Nursing principles. Inter-Personnel relationships. Bandaging -Basic turms, bandaging extremities, Triangular bandages and their application. Nursing Position, Bed making, prone, lateral, dorsal, dorsal re-cumbent, Fowler's positions, comfort measures, Aids and rest and sleep.

Recording of body temperature, respiration and pulse 0. Simple aseptic technique, sterlization and disinfection 0.

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